Immune checkpoint inhibitors (ICIs) are among the most effective classes of cancer immunotherapies yet only a small minority of patients derive clinical benefit. We are investigating the use of multi-spectral paired-agent imaging (mPAI) to quantify available PD-1 and PD-L1 receptor concentrations for the therapeutic binding of anti-PD-1 checkpoint inhibitors.
Paired Agent Imaging (PAI) is a fluorescence imaging technique where a targeted probe is co-administered with an untargeted probe. PAI has been successfully demonstrated in a pre-clinical setting and its clinical translation is in progress. The tissue distribution and excretion of the two fluorescent dyes, ABY-029 and IRDY680LT, must display similar kinetics in order for the PAI model to hold. To study the excretion of the dyes, plasma studies need to be conducted to examine the presence of fluorescence in vivo over a select period of time. The current method of measuring plasma fluorescence involves centrifuging blood to isolate plasma and them measuring on a fluorometer which can be time consuming and inefficient. In this study, we examine multiple methods for visualizing and quantifying plasma fluorescence using blood and plasma phantoms at multiple concentrations. The phantom fluorescence was measured using the Pearl Imaging system and the Fluoromax-3. We have determined that imaging blood directly in a fluorescence imaging system provides the same information as plasma alone.
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