Dr. Zhe Pei Profile

Dr. Zhe Pei

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Publications (4)

Proceedings Article | 6 March 2023 Presentation + Paper

Amyloid beta and tau protein metabolic changes detected by visible resonance Raman scattering microscopy in Alzheimer's disease mouse model

Lingyan Shi, Yan Zhou, Binlin Wu, Ke Zhu, Shengjia Zhang, Susie Boydston-White, Lin Zhang, Zhe Pei, Thien An Nguyen, Cheng-Hui Liu, Robert Alfano

Proceedings Volume 12373, 123730B (2023) https://doi.org/10.1117/12.2652913

KEYWORDS: Proteins, Raman spectroscopy, Brain tissue, Brain, Biological samples, Alzheimer disease, Tissues, Thalamus, Vibration, Spectroscopy

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Alzheimer’s disease (AD) pathogenesis is widely believed to be associated with the production and deposition of the β-amyloid peptide (Aβ) and neurofibrillary tangles (NFTs) which are composed of a highly-phosphorylated form of the microtubule-associated protein tau. Based on the above hypothesis, there are currently no sufficiently effective technologies and drugs for early detection and treatment of AD. Even the most promising new drug Lecanemab that is based on an anti-amyloid monoclonal antibody therapy, has only partially slowed down the cognitive performance of patients with mild impairment caused by Alzheimer's disease. The main symptoms of AD brain tissue lesions in patients are the deposition of β-amyloid peptide and the hyperphosphorylation of tau protein, which aggregates the microtubule structure of neurons. Therefore, Aβ deposition and hyperphosphorylation of Tau are important pathological biomarkers of Alzheimer's disease. Therefore, the main targets of research for AD prevention, detection and pharmaceuticals are still Aβ and Tau protein. The aim of this study was to detect the changes of Aβ and Tau proteins in the mouse brain tissue with AD and control samples using Visible Resonance Raman (VRR) spectroscopic technology. An attempt was made to develop criteria for the detection of early AD lesions by optical spectroscopy technology. The VRR spectra of AD, the control mouse brain tissues, and Aβ and Tau proteins were recorded and analyzed. The AD and the control mouse brain tissue samples were selected from the thalamus, frontal lobe cortex and hippocampus brain areas. VRR technology with high spatial resolution and the resonance-enhanced features of certain protein molecules is first used in this study to detect and characterize the changes of Aβ and Tau proteins in AD mouse brain model. The optical spectroscopy biomarkers of AD and Control brain tissue were identified in fingerprint and the high-wavenumber regions. The Raman spectra of the secondary structure of protein in amide (I-II-III-B-A) are detected and analyzed. The results indicate that the intensity of Amide I decreased at the 1666 cm^-1 corresponding to the β-sheet structure, and the intensity of the amide III bands (1220- 1320 cm^-1) increased in all AD brain tissues. It was also observed that the Raman peaks of 1448 and 980 cm^-1 related to the abundance of proline, serine, and threonine at tau phosphorylation sites were significantly enhanced in the frontal lobe cortex and hippocampus of AD brain tissues. The intensity ratio biomarker of high phosphorylation in the high wavenumber range from 2898 to 2932 cm-1 increased in all AD brain tissues. Changes of protein secondary conformation and abnormally phosphorylated tau or tauopathies were observed. In summary, VRR is a sensitive tool for characterizing protein structural changes and monitoring the tau phosphorylation. It may potentially be used for early detection of AD.

Proceedings Article | 2 March 2022 Presentation + Paper

Visible resonance Raman spectroscopic ex vivo studies of mouse brain and organs

Zhe Pei, Binlin Wu, Lingyan Shi, Lin Zhang, Susie Boydston-White, Cheng-Hui Liu, Robert Alfano

Proceedings Volume 11954, 1195402 (2022) https://doi.org/10.1117/12.2609810

KEYWORDS: Brain, Raman spectroscopy, Tissues, Proteins, Spectroscopy, Head, Visible radiation, Neurons, Molecules, Axons

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Proceedings Article | 17 February 2017 Paper

Resonance Raman of BCC and normal skin

Cheng-hui Liu, Vidyasagar Sriramoju, Susie Boydston-White, Binlin Wu, Chunyuan Zhang, Zhe Pei, Laura Sordillo, Hugh Beckman, Robert Alfano

Proceedings Volume 10060, 100601B (2017) https://doi.org/10.1117/12.2254628

KEYWORDS: Proteins, Cancer, Skin cancer, Biopsy, Statistical analysis

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SPIE Journal Paper | 21 December 2016 Open Access

Vulnerable atherosclerotic plaque detection by resonance Raman spectroscopy

Cheng-hui Liu, Susie Boydston-White, Arel Weisberg, Wubao Wang, Laura Sordillo, Adler Perotte, Vincent Tomaselli, Peter Sordillo, Zhe Pei, Lingyan Shi, Robert Alfano

JBO, Vol. 21, Issue 12, 127006, (December 2016) https://doi.org/10.1117/12.10.1117/1.JBO.21.12.127006

KEYWORDS: Raman spectroscopy, Tissues, Proteins, Virtual point source, Confocal microscopy, Photography, Collagen, Arteries, Absorption, Molecules

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