Design and realization of highly stable porous silicon optical biosensor based on proteins from extremophiles

Luca De Stefano; Lucia Rotiroti; Ilaria Rea; Edoardo De Tommasi; Annalisa Vitale; Mosè Rossi; Ivo Rendina; Sabato D'Auria

doi:10.1117/12.722389

16 May 2007 Design and realization of highly stable porous silicon optical biosensor based on proteins from extremophiles

Luca De Stefano, Lucia Rotiroti, Ilaria Rea, Edoardo De Tommasi, Annalisa Vitale, Mosè Rossi, Ivo Rendina, Sabato D'Auria

Author Affiliations +

Proceedings Volume 6585, Optical Sensing Technology and Applications; 658517 (2007) https://doi.org/10.1117/12.722389
Event: International Congress on Optics and Optoelectronics, 2007, Prague, Czech Republic

Abstract

The interaction between an analyte and a biological recognition system is normally detected in biosensors by the transducer element which converts the molecular event into a measurable effect, such as an electrical or optical signal. Porous silicon microstructures have unique optical and morphological properties that can be exploited in biosensing. The large specific surface area (even greater than 500 m²/cm³) and the resonant optical response allow detecting the effect of a change in refractive index of liquid solutions, which interact with the porous matrix, with very high sensitivity. Moreover, the porous silicon surface can be chemically modified to link the bioprobe which recognize the target analytes, in order to enhance the selectivity and specificity of the sensor device. The molecular probe we used was purified by an extremophile organism, Thermococcus litoralis: the protein is very stable in a wide range of temperatures even if with different behavior respect to the interaction with the ligand.

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Luca De Stefano, Lucia Rotiroti, Ilaria Rea, Edoardo De Tommasi, Annalisa Vitale, Mosè Rossi, Ivo Rendina, and Sabato D'Auria "Design and realization of highly stable porous silicon optical biosensor based on proteins from extremophiles", Proc. SPIE 6585, Optical Sensing Technology and Applications, 658517 (16 May 2007); https://doi.org/10.1117/12.722389

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