Dr. Michael A. Choma Profile

Dr. Michael A. Choma

SPIE Involvement:

Author | Editor

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Publications (36)

SPIE Journal Paper | 30 January 2019 Open Access

Redox imaging and optical coherence tomography of the respiratory ciliated epithelium

Daniel Gil, Joe Sharick, Sophie Mancha, Ute Gamm, Michael Choma, Melissa Skala

JBO, Vol. 24, Issue 01, 010501, (January 2019) https://doi.org/10.1117/12.10.1117/1.JBO.24.1.010501

KEYWORDS: Optical coherence tomography, Cyanide, Particles, Mode conditioning cables, Cartilage, Inflammation, Coherence imaging, Optical imaging, Bioalcohols, In vivo imaging

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Proceedings Article | 14 March 2018 Presentation

Optical coherence tomography and autofluorescence microscopy of respiratory ciliated epithelia (Conference Presentation)

Daniel Gil, Joe Sharick, Ute Gamm, Michael Choma, Melissa Skala

Proceedings Volume 10470, 1047005 (2018) https://doi.org/10.1117/12.2290576

KEYWORDS: Optical coherence tomography, Mode conditioning cables, Particles, Pulmonary disorders, Microscopy, Velocimetry, Microfluidics, Chronic obstructive pulmonary disease, Cystic fibrosis, Optical microscopy

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Proceedings Article | 24 April 2017 Presentation

Coherence switching of a vertical-cavity semiconductor-laser for multimode biomedical imaging (Conference Presentation)

Hui Cao, Sebastian Knitter, Changgeng Liu, Brandon Redding, Mustafa Kezar Khokha, Michael Andrew Choma

Proceedings Volume 10075, 1007502 (2017) https://doi.org/10.1117/12.2251513

KEYWORDS: Semiconductor lasers, Spatial coherence, Coherence imaging, Switching, Semiconductors, Heart, Biomedical optics, Modes of laser operation, Coherence (optics), Speckle

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Proceedings Article | 21 April 2017 Presentation

Intracavity frequency-doubled degenerate laser (Conference Presentation)

Hui Cao, Seng Fatt Liew, Sebastian Knitter, Sascha Weiler, Jesus Monjardin-Lopez, Mark Ramme, Brandon Redding, Michael Choma

Proceedings Volume 10088, 1008807 (2017) https://doi.org/10.1117/12.2251503

KEYWORDS: Laser development, Spatial coherence, Visible radiation, Speckle, Second-harmonic generation, Imaging spectroscopy, Laser applications, Infrared imaging, Infrared lasers, Luminescence

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Proceedings Article | 19 April 2017 Presentation

Quantification and visualization of injury and regeneration in the developing ciliated epithelium using quantitative flow imaging and speckle variance optical coherence tomography (Conference Presentation)

Ute Gamm, Brendan Huang, Emily Mis, Mustafa Khokha, Michael Choma

Proceedings Volume 10043, 100430O (2017) https://doi.org/10.1117/12.2255756

KEYWORDS: Injuries, Optical coherence tomography, Visualization, Speckle, Speckle imaging, Lung, Calcium, 3D image processing, Imaging systems, Pulmonary disorders

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Premature infants are at a high risk for respiratory diseases owing to an underdeveloped respiratory system that is very susceptible to infection and inflammation. One aspect of respiratory health is the state of the ciliated respiratory epithelium which lines the trachea and bronchi. The ciliated epithelium is responsible for trapping and removing pathogens and pollutants from the lungs and an impairment of ciliary functionality can lead to recurring respiratory infections and subsequent lung damage. Mechanisms of cilia-driven fluid flow itself but also factors influenced by development like ciliary density and flow generation are incompletely understood. Furthermore, medical interventions like intubation and accidental aspiration can lead to focal or diffuse loss of cilia and disruption of flow. In this study we use two animal models, Xenopus embryo and ex vivo mouse trachea, to analyze flow defects in the injured ciliated epithelium. Injury is generated either mechanically with a scalpel or chemically by calcium chloride (CaCl2) shock, which efficiently but reversibly deciliates the embryo skin. In this study we used optical coherence tomography (OCT) and particle tracking velocimetry (PTV) to quantify cilia driven fluid flow over the surface of the Xenopus embryo. We additionally visualized damage to the ciliated epithelium by capturing 3D speckle variance images that highlight beating cilia. Mechanical injury disrupted cilia-driven fluid flow over the injured site, which led to a reduction in cilia-driven fluid flow over the whole surface of the embryo (n=7). The calcium chloride shock protocol proved to be highly effective in deciliating embryos (n=6). 3D speckle variance images visualized a loss of cilia and cilia-driven flow was halted immediately after application. We also applied CaCl2-shock to cultured ex vivo mouse trachea (n=8) and found, similarly to effects in Xenopus embryo, an extensive loss of cilia with resulting cessation of flow. We investigated the regeneration of the ciliated epithelium after an 8 day incubation period, and found that cilia had regrown and flow was completely restored. In conclusion, OCT is a valuable tool to visualize injury of the ciliated epithelium and to quantify reduction of generated flow. This method allows for systematic investigation of focal and diffuse injury of the ciliated epithelium and the assessment of mechanisms to compensate for loss of flow.

Proceedings Article | 19 April 2017 Presentation

Evaluation of structural remodeling of the atria with OCT in a chronic rat model of myocardial infarction (Conference Presentation)

Melissa Eberle, Stephanie Thorn, Lawerence Young, Daniel Pfau, Jeffrey Madwed, Kersten Small, Michael Kilmas, Michael Choma, Albert Sinusas

Proceedings Volume 10042, 1004206 (2017) https://doi.org/10.1117/12.2252954

KEYWORDS: Natural surfaces, Optical coherence tomography, Signal attenuation, Atrial fibrillation, Tissue optics, Fractal analysis, Arteries, Surgery, Tissues, Collagen

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Proceedings Article | 19 April 2017 Presentation

Functional optical imaging of tracheal health (Conference Presentation)

Daniel Gil, Joe Sharick, Ute Gamm, Michael Choma, Melissa Skala

Proceedings Volume 10041, 100410O (2017) https://doi.org/10.1117/12.2252226

KEYWORDS: Fiber optics, In vivo imaging, Medicine, Optical imaging, Mode conditioning cables, Luminescence, Cyanide, Inflammation, Sodium, Hydrogen

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Proceedings Article | 19 April 2017 Presentation

Visualization of ex vivo human ciliated epithelium and induced flow using optical coherence tomography (Conference Presentation)

Yuye Ling, Uta Gamm, Xinwen Yao, Emilio Arteaga-Solis, Charles Emala, Michael Choma, Christine Hendon

Proceedings Volume 10041, 1004106 (2017) https://doi.org/10.1117/12.2253045

KEYWORDS: Image processing, Optical coherence tomography, Imaging systems, Visualization, Speckle, Microorganisms, Tissues, Oxygen, 3D image processing, Image resolution

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Proceedings Article | 19 April 2017 Presentation

Quantification and visualization of injury and regeneration to the ciliated epithelium using quantitative flow imaging and speckle variance optical coherence tomography (Conference Presentation)

Ute Gamm, Brendan Huang, Emily Mis, Mustafa Khokha, Michael Choma

Proceedings Volume 10041, 1004104 (2017) https://doi.org/10.1117/12.2252405

KEYWORDS: 3D image processing, Defense and security, Lung, Injuries, Optical coherence tomography, Visualization, Speckle, Speckle imaging, Medicine, Calcium

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Mucociliary flow is an important defense mechanism in the lung to remove inhaled pathogens and pollutants. A disruption of ciliary flow can lead to respiratory infections. Even though patients in the intensive care unit (ICU) either have or are very susceptible to respiratory infections, mucociliary flow is not well understood in the ICU setting. We recently demonstrated that hyperoxia, a consequence of administering supplemental oxygen to a patient in respiratory failure, can lead to a significant reduction of cilia-driven fluid flow in mouse trachea. There are other factors that are relevant to ICU medicine that can damage the ciliated tracheal epithelium, including inhalation injury and endotracheal tube placement. In this study we use two animal models, Xenopus embryo and ex vivo mouse trachea, to analyze flow defects in the injured ciliated epithelium. Injury is generated either mechanically with a scalpel or chemically by calcium chloride (CaCl2) shock, which efficiently but reversibly deciliates the embryo skin. In this study we used optical coherence tomography (OCT) and particle tracking velocimetry (PTV) to quantify cilia driven fluid flow over the surface of the Xenopus embryo. We additionally visualized damage to the ciliated epithelium by capturing 3D speckle variance images that highlight beating cilia. Mechanical injury disrupted cilia-driven fluid flow over the injured site, which led to a reduction in cilia-driven fluid flow over the whole surface of the embryo (n=7). The calcium chloride shock protocol proved to be highly effective in deciliating embryos (n=6). 3D speckle variance images visualized a loss of cilia and cilia-driven flow was halted immediately after application. We also applied CaCl2-shock to cultured ex vivo mouse trachea (n=8) and found, similarly to effects in Xenopus embryo, an extensive loss of cilia with resulting cessation of flow. We investigated the regeneration of the ciliated epithelium after an 8 day incubation period, and found that cilia had regrown and flow was completely restored. In conclusion, OCT is a valuable tool to visualize injury of the ciliated epithelium and to quantify reduction of generated flow. This method allows for systematic investigation of focal and diffuse injury of the ciliated epithelium and the assessment of mechanisms to compensate for loss of flow.

Proceedings Article | 27 April 2016 Presentation

High-speed line-scanning confocal holographic microscopy for quantitative phase imaging (Conference Presentation)

Changgeng Liu, Sebastian Knitter, Zhilong Cong, Ikbal Sencan, Hui Cao, Michael Choma

Proceedings Volume 9718, 971820 (2016) https://doi.org/10.1117/12.2212033

KEYWORDS: Confocal microscopy, Holography, Microscopes, Line scan image sensors, Phase imaging, Microscopy, Digital holography, Medicine, Reflectivity, Cameras

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Proceedings Article | 27 April 2016 Presentation

OCT-based three-dimensional, three vector component imaging of cilia-driven fluid flow for developmental biology (Conference Presentation)

Brendan Huang, Kevin Zhou, Ute Gamm, Vineet Bhandari, Mustafa Khokha, Michael Choma

Proceedings Volume 9716, 97160J (2016) https://doi.org/10.1117/12.2211601

KEYWORDS: 3D image processing, Biology, Particles, Velocimetry, 3D metrology, Skin, Calibration, Medicine, Velocity measurements, Reconstruction algorithms

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Proceedings Article | 27 April 2016 Presentation

OCT imaging of craniofacial anatomy in xenopus embryos (Conference Presentation)

Engin Deniz, Stephan Jonas, John Griffin, Michael Hooper, Michael Choma, Mustafa Khokha

Proceedings Volume 9716, 97160F (2016) https://doi.org/10.1117/12.2213448

KEYWORDS: Optical coherence tomography, Genetics, Biological research, Nondestructive evaluation, 3D modeling, Animal model studies, 3D scanning, 3D imaging standards, Visualization, Excel

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Proceedings Article | 27 April 2016 Presentation

Towards all-optical quantification of force- and power-based performance metrics in cilia-driven fluid flow physiology (Conference Presentation)

Brendan Huang, Mustafa Khokha, Michael Loewenberg, Michael Choma

Proceedings Volume 9691, 969119 (2016) https://doi.org/10.1117/12.2212563

KEYWORDS: Physiology, Tissues, Heart, Velocimetry, Cell mechanics, Resistance, Performance modeling, Optical coherence tomography, Endoscopy, Optical microscopy

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Proceedings Article | 26 April 2016 Presentation

OCT-based quantification of flow velocity, shear force, and power generated by a biological ciliated surface (Conference Presentation)

Brendan Huang, Mustafa Khokha, Michael Loewenberg, Michael Choma

Proceedings Volume 9697, 969729 (2016) https://doi.org/10.1117/12.2214793

KEYWORDS: Optical coherence tomography, Physiology, Acquisition tracking and pointing, Cell mechanics, Reflectivity, Animal model studies, Systems modeling, Coherence (optics), Biomedical optics, Current controlled current source

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Proceedings Article | 26 April 2016 Presentation

Particle streak velocimetry-OCT (PSV-OCT): a novel method for multi-vector component velocimetry of microscale flow physiology (Conference Presentation)

Kevin Zhou, Brendan Huang, Ute Gamm, Vineet Bhandari, Mustafa Khokha, Michael Choma

Proceedings Volume 9697, 96970F (2016) https://doi.org/10.1117/12.2214794

KEYWORDS: Particles, Velocimetry, Optical coherence tomography, Physiology, Microfluidics, Animal model studies, Microfluidic imaging, Image acquisition, Biological research, 3D modeling

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Proceedings Article | 6 April 2016 Presentation + Paper

Optical metabolic imaging for monitoring tracheal health

Joe Sharick, Daniel Gil, Michael Choma, Melissa Skala

Proceedings Volume 9711, 971108 (2016) https://doi.org/10.1117/12.2212779

KEYWORDS: Optical imaging, Two photon excitation microscopy, Proteins, Fluorescence lifetime imaging, FRET and FLIM microscopy, Multiphoton fluorescence microscopy, Fluorescence photon counting, Fluorescent proteins, Tissues, Tissue optics, Natural surfaces, Diagnostics, Medicine, Lung

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SPIE Journal Paper | 26 August 2015 Open Access

Quantifying hyperoxia-mediated damage to mammalian respiratory cilia-driven fluid flow using particle tracking velocimetry optical coherence tomography

Ute Gamm, Brendan Huang, Mansoor Syed, Xuchen Zhang, Vineet Bhandari, Michael Choma

JBO, Vol. 20, Issue 08, 080505, (August 2015) https://doi.org/10.1117/12.10.1117/1.JBO.20.8.080505

KEYWORDS: Hyperoxia, Particles, Oxygen, Optical coherence tomography, Velocimetry, Optical tracking, Modulation, Acquisition tracking and pointing, Body temperature, Biomedical optics

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SPIE Journal Paper | 9 March 2015 Open Access

Quantitative optical coherence tomography imaging of intermediate flow defect phenotypes in ciliary physiology and pathophysiology

Brendan Huang, Ute Gamm, Stephan Jonas, Mustafa Khokha, Michael Choma

JBO, Vol. 20, Issue 03, 030502, (March 2015) https://doi.org/10.1117/12.10.1117/1.JBO.20.3.030502

KEYWORDS: Proteins, Optical coherence tomography, Coherence imaging, Physiology, Optical imaging, Pathophysiology, Genetics, Particles, Microfluidics, Optical tracking

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Proceedings Article | 29 March 2013 Paper

Quantification of microfluidic dye mixing using front line tracking in curvature scale space

Stephan Jonas, Elaine Zhou, Brendan Huang, Michael Choma, Thomas Deserno

Proceedings Volume 8672, 867206 (2013) https://doi.org/10.1117/12.2006888

KEYWORDS: Image segmentation, Microfluidics, Image processing, Interfaces, Diffusion, Image visualization, Gaussian filters, RGB color model, Video, Optical inspection

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Proceedings Article | 9 February 2012 Paper

Entropy-based measures of in vivo cilia-driven microfluidic mixing derived from quantitative optical imaging

Kenny Chandrasekera, Stephan Jonas, Dipankan Bhattacharya, Mustafa Khokha, Michael Choma

Proceedings Volume 8207, 82073K (2012) https://doi.org/10.1117/12.906428

KEYWORDS: Microfluidics, In vivo imaging, Microfluidic imaging, Optical imaging, Pulmonary disorders, Particles, Natural surfaces, Visualization, RGB color model, Diagnostics

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Proceedings Article | 12 September 2011 Paper

Spatial coherence of random laser emission

B. Redding, M. Choma, H. Cao

Proceedings Volume 8095, 80950P (2011) https://doi.org/10.1117/12.893091

KEYWORDS: Spatial coherence, Random lasers, Scattering, Laser scattering, Light scattering, Coherence (optics), Visibility, Optical spheres, Speckle, Spherical lenses

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SPIE Journal Paper | 1 September 2010 Open Access

Heart wall velocimetry and exogenous contrast-based cardiac flow imaging in Drosophila melanogaster using Doppler optical coherence tomography

Michael Choma, Melissa Suter, Benjamin Vakoc, Brett Bouma, Gary Tearney

JBO, Vol. 15, Issue 05, 056020, (September 2010) https://doi.org/10.1117/12.10.1117/1.3503418

KEYWORDS: Heart, Doppler effect, Optical coherence tomography, Doppler tomography, Velocimetry, Particles, Video, Biology, Scattering, Imaging systems

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SPIE Journal Paper | 1 July 2007 Open Access

Spectral domain phase microscopy for local measurements of cytoskeletal rheology in single cells

Emily McDowell, Audrey Ellerbee, Michael Choma, Brian Applegate, Joseph Izatt

JBO, Vol. 12, Issue 04, 044008, (July 2007) https://doi.org/10.1117/12.10.1117/1.2753755

KEYWORDS: Data modeling, Magnetism, Microscopy, Cytoskeletons, Reflectors, Reflection, Interferometry, Optical coherence tomography, Interfaces, Motion models

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SPIE Journal Paper | 1 March 2006 Open Access

Doppler flow imaging of cytoplasmic streaming using spectral domain phase microscopy

Michael Choma, Audrey Ellerbee, Siavash Yazdanfar, Joseph Izatt

JBO, Vol. 11, Issue 02, 024014, (March 2006) https://doi.org/10.1117/12.10.1117/1.2193167

KEYWORDS: Doppler effect, Interference (communication), Optical coherence tomography, Reflectors, Signal to noise ratio, Doppler tomography, Microscopy, Interferometry, Reflectivity, Interferometers

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SPIE Journal Paper | 1 November 2005 Open Access

Heterodyne swept-source optical coherence tomography for complete complex conjugate ambiguity removal

Anjul Maheshwari, Michael Choma, Joseph Izatt

JBO, Vol. 10, Issue 06, 064005, (November 2005) https://doi.org/10.1117/12.10.1117/1.2136147

KEYWORDS: Heterodyning, Optical coherence tomography, Interferometry, Reflectors, Imaging systems, Demodulation, Image segmentation, Homodyne detection, Signal attenuation, Signal to noise ratio

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SPIE Journal Paper | 1 July 2005 Open Access

Swept source optical coherence tomography using an all-fiber 1300-nm ring laser source

Michael Choma, Kevin Hsu, Joseph Izatt

JBO, Vol. 10, Issue 04, 044009, (July 2005) https://doi.org/10.1117/12.10.1117/1.1961474

KEYWORDS: Optical coherence tomography, Laser sources, Signal to noise ratio, Cornea, Image segmentation, Interferometry, Reflectors, Photodiodes, Interferometers, In vivo imaging

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Proceedings Article | 13 April 2005 Paper

Characterizing cellular contractility and cytoplasmic flow using spectral domain phase microscopy

Audrey Ellerbee, Michael Choma, Emily McDowell, Anthony Creazzo, Joseph Izatt

Proceedings Volume 5690, (2005) https://doi.org/10.1117/12.592809

KEYWORDS: Microscopy, Reflectors, Optical coherence tomography, Doppler effect, Video microscopy, Signal to noise ratio, Microscopes, Video, Doppler tomography, Organisms

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Proceedings Article | 13 April 2005 Paper

Heterodyne swept-source optical coherence tomography for complete complex conjugate ambiguity removal

Anjul Maheshwari, Michael Choma, Joseph Izatt

Proceedings Volume 5690, (2005) https://doi.org/10.1117/12.592867

KEYWORDS: Heterodyning, Reflectors, Optical coherence tomography, Interferometry, Homodyne detection, Image segmentation, Imaging systems, Bragg cells, Spectral resolution, Fourier transforms

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Proceedings Article | 24 March 2005 Paper

In vivo imaging of the Drosophila Melanogaster heart using a novel optical coherence tomography microscope

Susan Izatt, Michael Choma, Steven Israel, Robert Wessells, Rolf Bodmer, Joseph Izatt

Proceedings Volume 5701, (2005) https://doi.org/10.1117/12.596122

KEYWORDS: Heart, Optical coherence tomography, Microscopes, In vivo imaging, Genetics, Imaging systems, Coherence imaging, Real time imaging, 3D acquisition, 3D image processing

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Proceedings Article | 24 March 2005 Paper

Spectral domain phase microscopy: a new tool for measuring cellular dynamics and cytoplasmic flow

Emily McDowell, Michael Choma, Audrey Ellerbee, Joseph Izatt

Proceedings Volume 5701, (2005) https://doi.org/10.1117/12.601505

KEYWORDS: Optical coherence tomography, Microscopy, Doppler effect, Magnetism, Reflectors, Data modeling, Interferometry, Video microscopy, Interferometers, Reflection

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Proceedings Article | 1 July 2004 Paper

Protein-based molecular contrast optical coherence tomography

Changhuei Yang, Michael Choma, Laura Lamb, John Simon, Joseph Izatt

Proceedings Volume 5316, (2004) https://doi.org/10.1117/12.531353

KEYWORDS: Optical coherence tomography, Proteins, Absorption, Chromophores, In vivo imaging, Luminescence, Image processing, Microscopy, Optical switching, Scattering

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Proceedings Article | 1 July 2004 Paper

Instantaneous complex spectral domain OCT using 3x3 fiber couplers

Marinko Sarunic, Michael Choma, Changhuei Yang, Joseph Izatt

Proceedings Volume 5316, (2004) https://doi.org/10.1117/12.531422

KEYWORDS: Optical coherence tomography, Sensors, Interferometers, Fiber couplers, Interferometry, Spectrometers, Signal detection, Reflectors, Mirrors, Photodiodes

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Proceedings Article | 2 October 2003 Paper

Molecular contrast in optical coherence tomography using a pump-probe technique and a optical switch suppression technique

Changhuei Yang, K. Rao, Michael Choma, Siavash Yazdanfar, Andrew Rollins, Joseph Izatt

Proceedings Volume 5140, (2003) https://doi.org/10.1117/12.501007

KEYWORDS: Optical coherence tomography, Absorption, Optical switching, Scattering, Imaging systems, Ultraviolet radiation, Molecules, Proteins, Molecular spectroscopy, Luminescence

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Proceedings Article | 8 July 2003 Paper

Wavelet and Eigenfrequency spectral analysis of color Doppler optical coherence tomography

Michael Choma, Siavash Yazdanfar, Joseph Izatt

Proceedings Volume 4956, (2003) https://doi.org/10.1117/12.479040

KEYWORDS: Doppler tomography, Optical coherence tomography, Wavelets, Blood vessels, Interferometry, Fourier transforms, Wavelet transforms, Doppler effect, In vivo imaging, Data modeling

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Proceedings Article | 8 July 2003 Paper

Molecular contrast in optical coherence tomography using a pump-probe technique

K. Rao, Michael Choma, Siavash Yazdanfar, Andrew Rollins, Joseph Izatt

Proceedings Volume 4956, (2003) https://doi.org/10.1117/12.479039

KEYWORDS: Optical coherence tomography, Absorption, Raman scattering, Spectroscopy, Luminescence, Light scattering, Scattering, Tissue optics, Transient nonlinear optics, Interfaces

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Proceedings Article | 8 July 2003 Paper

Three-dimensional OCT imaging of the embryonic chick heart

Michael Choma, T. Yelbuz, Lars Thrane, Margaret Kirby, Joseph Izatt

Proceedings Volume 4956, (2003) https://doi.org/10.1117/12.479042

KEYWORDS: Optical coherence tomography, Heart, 3D image processing, 3D modeling, Animal model studies, Magnetic resonance imaging, 3D image reconstruction, Biomedical optics, Image resolution, Imaging systems

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